HCV的Core-NS3嵌合抗原在大肠杆菌中的表达、纯化及初步应用

Expression,purification of HCV Core-NS3 chimeric antigen in E.coli and its application

用已经构建的含有HCV的Core-NS3(C33 c)嵌合基因的表达质粒pGEX TL1-2在大肠杆菌中进行了高效表达,表达产物纯化后通过SDS-PAGE、W estern-b lot、ELISA等一系列鉴定试验进行了分析,结果表明,该嵌合抗原具有高度特异性和良好的抗原活性,为研制诊断用HCV抗原奠定基础。

The constructed expression plasmid pGEX TL1-2 which contains chimeric gene of HCV Core-NS3 （ C33c ） was highly expressed. The purified recombinant protein was analyzed by SDS-PAGE, Western-blot and indirect ELISA, respectively. The prepared chimeric antigen showed high specificity and good antigenicity, and it laid a foundation for studying and developing of HCV diagnostic antigens.