63例慢性乙肝e抗原阴性HBV基因多态性检测结果报告

Detection gene polymorphism of HBV in 63 cases of eAg(-) chronic HBV infections

用地高辛标记引物酶显色法,检测了63例e抗原阴性慢性肝炎HBV基因多态性。结果突变率为53.9%(34/63)。前C/C区1896位突变率最高为49.2%(31/63),1814位38.1%(24/63);BCP区1762位、1764位均为39.7%(25/63),552位突变率为14.3%(9/63)。该检测方法灵敏度高,简便易行。严格控制杂交温度及显色温度是检测操作的关键。

Detection of HBV gene polymorphism was carried out by using of enzyme coloration with digoxin-labeled primers in 63 cases of eAg （ - ） chronic HBV infections. And the detected mutation rate was 53.9% （ 34/63 ）. The mutation rate in 1896th position of pre-c/c region was the highest as 49.2% （31/63） ; in 1814th was 38.1% （24/63） ;the mutation rate in 1762th and 1764th in BCP region were the same as 39.7% （25/63）. The mutation rate in 552th position in P region was 14.3% （ 9/63 ）. The method is more sensitive and easily operated in clinical practice. The key points are strictly control of temperature both in the hybridization and colour development in the detection.