姜黄素抑制人血管内皮细胞增殖的研究

Inhibition effect of curcumin on proliferation of human umbilical vein endothelial cells

目的观察姜黄素对体外培养人脐静脉内皮细胞（HUVEC）增殖和凋亡的影响，寻找辅助治疗和预防翼状胬肉复发的新方法。方法用0～160gμmol／L姜黄素作用体外培养的HUVEC，24～96h后观察不同浓度姜黄素对HUVEC的影响。MTY法及免疫组织化学染色增殖细胞核抗原（PCNA）检测细胞生长活性，流式细胞仪测定细胞周期时相变化。结果在40～160μmol／L浓度作用24～72h范围内，姜黄素可剂量和时间依赖性的抑制HUVEC增殖（P＜0．05）。20，40，80，160μmol／L姜黄素作用24h后，流式细胞仪检测结果发现，姜黄素作用组均出现典型的亚二倍体凋亡峰，G0／G1期细胞百分比上升（P＜0．05），S期细胞百分比下降（P＜0．05），说明细胞阻滞于G0／G1期。当姜黄素的浓度在40～160μmol／L范围内能浓度依赖性地抑制细胞表达PCNA（P＜0．05）。结论姜黄素可显著抑制HUVEC增殖，使细胞周期停滞于G0／G1期，并诱导其凋亡，作用呈时同和剂量依赖性。提示姜黄素通过抑制新生血管治疗翼状胬肉具有潜在的应用前景。

Objective To investigate the effect of cuurcumin on proliferation and apoptosis of human umbilical vein endothelial cell （HUVEC） in culture and search for a new method to prevent the recurrence after pterygium surgery. Methods HUVEC was incubated with 0 ～ 160μmol/L curcumin for 24 ～ 96h. The MTT method was used to assay the biologic activities of curcumin in different time and different doses. The expression of proliferating cell nuclear antigen（PCNA） in each group was detected by immunohistochemistry method. The cell cycle distribution was detected by flow cytometic analysis. Results Administration of 20 ～ 80μumol/L curcumin for 24 ～ 72h could significantly inhibit HUVEC proliferation in a dose-and time-dependent manner （ P 〈 0. 05 ）. After treated with curcumin at different concentrations of 20,40,80, 160μmol/L for 24 h, there was significant sub-G1 peak using FCM. The number of HUVEC in GO/G1 phase increased while in S phase decreased（ P 〈0.05） ,makes it arrest in G0/G1 phase. Within 20 ～ 80μmol/L curcumin can inhibit the expression of PCNA in HUVEC in a dose-dependent manner（ P 〈 0.05 ）. Conclusion Curcumin significantly inhibits HUVEC proliferation, makes it arrest in G0/G1 phase and induces apeptosis of HUVEC, which effects are dose-and timedependent. We have reason to conclude that curcumin have a possible therapeutic potential in pterygium patient by the antineovascularization effect.