甲壳质套管在大鼠脊髓横断损伤修复中的应用

Researches on the application of biological chitin tube in spinal cord injury of rats

目的:探讨甲壳质生物套管在大鼠脊髓完全横断损伤中的应用价值。方法:16只雄性SD大鼠随机分为2组,行T8-9节段脊髓完全横断损伤,A组单纯横断脊髓作为对照,B组用甲壳质生物套管套接横断脊髓断端,术后1个月时处死取材,行HE、尼氏染色及免疫组织化学染色(NF200、GFAP)检查。结果:大鼠对甲壳质套管无明显排斥反应;B组可见断端脊髓纤维向套管间隙内生长,且脊髓头侧断端前角运动细胞数量明显多于A组(P<0.05),细胞处于活跃功能相,GFAP染色阳性面积A组明显大于B组(P<0.05),B组套管间隙内可见神经纤维丝(NF200)及GFAP染色阳性胶质细胞框架存在,A组断端间为较大空洞,无神经纤维及胶质细胞的存在。结论:甲壳质生物套管在大鼠脊髓损伤的修复过程中可为断端脊髓神经纤维生长提供间隙,这种套接材料及方式可作为研究和治疗脊髓损伤的模型和方法。

Objective：To investigate the application of biological chitin tube in spinal cord injury of rats. Method：Sixteen male SD rats which were divided into A and B groups underwent completely T8-9 transection injury.Ends of spinal cord were connected with biological chitin tube in group B,group A was as control. After one month,the rats were sariticed,and the ends of cord with HE and Nissl and immunohistochemical （NF200 ＆ GFAP） stain.Result：There was no rejection observed in the rats to the tube.In group B.tissues grew into the tube gaps;the number of motor neurons of anterior corn in proximal end were more than that in group A （P〈0.05）,neural fibers and neuroglia were found in the gaps.In group A.neither fibers nor neuroglia occured between the two ends of spinal cord.Conclusion： Biological chitin tube can offer the gap between the ruptured ends of spinal cord to make nerve regrow.So this tube can be used as a valuable model and method to study and treat the spinal cord injury.