摘要
目的:探讨维生素A对角膜移植排斥反应后结膜杯状细胞的影响。方法:建立大鼠角膜移植排斥反应模型,随机分3组:A、B、C组为SD-Wistar大鼠行同种异体角膜移植术,SD鼠为受体,Wistar鼠为供体,其中A组为空白对照组,B组为(维生素A)诺沛凝胶滴眼液组,C组为0.1%地塞米松滴眼液组,另设D组为正常眼组。术后不同时间裂隙灯显微镜记录及比较各组角膜移植排斥指数(RejectionIndex,RI)。通过结膜组织学切片HE、PAS染色,并运用显微图像分析系统对结膜上皮杯状细胞数量及形态进行分析。结果:观察期间检测结膜杯状细胞发现,A、B、C组杯状细胞数量均少于D组(P<0.01),A、B、C组内部比较,以C组数量为多,其次为B组,有差异有显著性(P<0.05)。结论:维生素A具有保护角膜移植引起的结膜杯状细胞减少的作用。
Purpose： To investigate the effect of oculotect gel and alteration of goblet cells of rat after corneal transplantation.
Methods： 48 SD rats were randomly divided into oculotect gel-treated, 0. 1% dexamethasone eye drops-treated, control groups and normal groups. After the rat model of heterograft-heterotopic graft rejection was made, slit-limp microscope and PAS stainings, light microscope and HE, PAS staining technique were used to observe the number and morphological difference of goblet cells in conjunctiva between operative groups and normal groups.
Results： The HE.PAS staining detection showed that the number of conjunctiva goblet cells in oculotect gel group.0.1% dexamethasone eye drops group and control groups is lower than that in normal group after surgery （P 〈 0.01 ）. The number of conjunctiva goblet cells in oculotect gel group and 0. 1% dexamethasone eye drops group is higher than that in control group （P 〈 0.05）. That number in 0.1% dexamethasone eye drops group is higher than that in oculotect gel group （P 〈 0.05）.
Conclusions： The results indicate that oculotect gel significantly inhibit the decrease of goblet ceils after corneal aUograft rejection in rats. Eye Sci 2006;22：229-232.
出处
《眼科学报》
2006年第4期229-232,243,共5页
Eye Science
