角质细胞生长因子对喉癌细胞株Hep-2及细胞外调节信号激酶活性及表达的影响

Effects of keratinocyte growth factor on the expression and activity of extracellular signal-regulated kinase in Hep-2 cell line

目的:观察角质细胞生长因子(keratinocytegrowthfactor,KGF)对喉癌细胞株Hep-2增殖及细胞外调节信号激酶(extracellularsignal-regulatedkinase,ERK)活性和表达的影响。方法:培养喉癌细胞株Hep-2,MTT比色法及流式细胞仪测定不同浓度KGF对细胞增殖的影响;免疫沉淀法纯化蛋白并用ERK1/2试剂盒测定ERK活性;Western-blot测定p-ERK表达。结果:KGF能显著促进细胞增殖,增强ERK活性及p-ERK表达。结论:KGF可以促使喉癌细胞株Hep-2增殖,其机制与增强ERK活性及p-ERK表达有关。

Objective：To observe effects of keratinocyte growth factor （KGF） on the proliferation of Hep 2 cell line; the activity and expression of extracellular signal regulated kinase （ERK）. Method： Cell line proliferation was measured by Flow eytometry and MTT assay; ERK activity was measured by ERK1/2 imrnuno-preeipitation kinase assay kit; and p ERK expression was assessed by Western blot, Result：KGF can improve the proliferation of Hep-2 cell line, The intracellular ERK activity and p-ERK expression were increased by different concentrations of KGF. Conclusion：KGF can stimulated proliferation of Hep-2 cell line with enhancing activity of ERK and expression of p-ERK.