花背蟾蜍胎肺发育中表皮生长因子受体的表达和定位作用

Expression of Epidermal Growth Factor Receptor(EGFR) in the Fetal Lungs of Bufo raddei

取花背蟾蜍(Bufo raddei)363、7、38、39期蝌蚪肺组织和幼蟾肺组织,进行常规石蜡切片,用免疫组化SP两步法检测EGFR的表达。观察了表皮生长因子受体(EGFR)在花背蟾蜍胎肺发育过程的表达特征,并探讨表皮生长因子(EGF)和转化生长因子α(TGF-α)通过与EGFR的作用,对花背蟾蜍胎肺形态发生和肺泡上皮成熟分化的作用。结果表明,36期,EGFR在肺网状隔膜上皮细胞处有表达;37期,肺网状隔膜处EGFR阳性表达很明显,在肺泡囊处表达呈弱阳性;38期,肺网状隔膜处EGFR的阳性表达变弱,在远端的肺泡囊上皮细胞处其阳性表达增强;39期,EGFR在肺泡囊上皮细胞处阳性表达最活跃,在网状隔膜处EGFR的表达很弱;幼蟾期,EGFR阳性反应主要定位在肺泡上皮细胞。结论是,在胎肺发育的不同时期,EGFR在上皮细胞的定位有迁移,免疫组化反应强弱也有差异,说明EGFR在胎肺不同发育阶段发挥不同的功能,它对肺泡上皮细胞的成熟分化有重要调节作用。

The expression of epidermal growth factor receptor（EGFR） in the lungs of tadpole and young toad（ Bufo raddei ） were examined by immuno-histochemistry. The results showed that EGFR was mainly localized in epithelium of the reticular septa at stage 36, and the staining reached a peak level at stage 37. At stage 38, EGFR was mainly localized in epithelium of alveolar sac, and the staining reached a peak level at stage 39. EGFR was mainly localized in alveoli epithelium in young toad lung. We conclude that the expression of EGFR changes during individual lung development,suggesting that it functions differently at various stages.