刺蒺藜对小鼠毛囊黑素细胞刺激素表达的影响

Effect of Tribulus terrestris extract on melanocyte-stimulating hormone expression in mouse hair follicles

目的观察刺蒺藜水提物对C57BL/6J小鼠毛囊黑素细胞刺激素表达的影响,探讨刺蒺藜在毛囊休眠期黑素细胞活化、增殖及移行至表皮过程中的作用。方法选择在离体实验中对酪氨酸酶有激活作用、对黑素细胞有增殖作用及在临床应用中对白癜风有治疗作用的中药刺蒺藜,用SPF级C57BL/6J小鼠为实验动物,采用免疫组织化学技术观察高剂量(相当于成人1g/(kg·d)刺蒺藜水提物灌服后小鼠毛囊黑素细胞刺激素水平的变化,并用χ2检验进行统计学分析。结果黑素细胞刺激素在试验组小鼠毛囊细胞中阳性表达率为75.00%,在对照组小鼠毛囊细胞中阳性表达率为18.75%,两者比较有显著性差异(P<0.01)。结论高剂量刺蒺藜水提物可以提高小鼠毛囊黑素细胞刺激素的表达水平,并可能通过上调毛囊黑素细胞刺激素表达这一途径来活化酪氨酸酶、促进毛囊休眠期黑素细胞的增殖、移行及黑素合成。

Objective TO observe the effect of Tribulus terrestris extract on melanocyte stimulating hormone （MSH） expression in C57BL/6J mouse hair follicles, and investigate the role of Tribulus terrestris extract in activation, proliferation, epidermal migration of dormant hair follicle melanocytes. Methods The aqueous extract of Tribulus terrestris was administered orally in specific pathogen-free C57BL/6J mouse at the daily dose equivalent to 1 g/l kg in adult human, and the expression and distribution of MSH in the mouse hair follicles was observed with immunohistochemistry. Results The positivity rate of MSH expression in the hair follicle melanocytes was 75% in mice treated with the extract, significantly higher than the rate of only 18.75% in the control group （P〈0.01）. Conclusion The aqueous extract of Tribulus terrestris can significantly increase MSH expression in the hair follicle melanocytes by activating tyrosinase activity and promoting melanocyte proliferation, melanine synthesis, and epidermal migration of dormant melanocytes.