血竭及其成分龙血素B影响背根神经节细胞钠离子通道的计算机模拟研究

A Computer Simulation Research for the Effects of Dragon's Blood And Its Component Loureirin B on Sodium Channel in Dorsal Root Ganglion Cells

应用膜片钳技术,观察中药血竭及其成分龙血素B对背根神经节细胞河豚毒素敏感型钠通道电流的影响。以Hodgkin-Huxley模型拟合实验资料,进行参数估计,并对药物作用前后的细胞膜动作电位进行计算机模拟。结果表明,m3h模型能较好地拟合河豚毒素敏感型钠通道电导;药物作用于钠通道后,半激活电压均向去极化方向偏移;药物作用后的细胞产生动作电位的阈强度提高。说明血竭及其成分龙血素B并非类似河豚毒素完全抑制河豚毒素敏感型钠通道电流。血竭可能通过其成分龙血素B影响通道的激活过程阻滞动作电位的产生,干预痛觉信息的中枢传入而产生镇痛效应。

Using patch clamp technique the effects of dragon＇s blood and its component loureirin B on tetrodotoxin-sensitive sodium channel currents in dorsal root ganglion cells were observed. The experimental data were simulated with Hodgkin-Huxley model and the corresponding parameters were estimated. In addition, computer-simulated neuron action potentials in the absence and presence of drugs were produced using Hodgkin- Huxley model. The results show that the conductance of tetrodotoxin-sensitive sodium channel was fitted with m^3h model well, the half-activated potentials of the sodium channel in the presence of drugs were shifted to the depolarizing direction and the threshold intensity of the cells in the presence of drugs was increased. These results demonstrate that dragon＇s blood and loureirin B did not resemble the tetrodotoxin which inhibited tetrodotoxinsensitive sodium channel currents completely. Perhaps the analgesic effects of dragon＇s blood were partly caused by loureirin B affecting the activation, blocking the action potential generation and interfering with the transmission of painful signals into the central nervous system.