Midkine在胰腺癌组织中的表达及临床意义

Clinical significance of Midkine expression in pancreatic carcinoma

目的探讨人胰腺癌组织中Midkine(MK)的表达及其与肿瘤细胞增殖的关系和意义。方法免疫组织化学SP法检测49例胰腺癌、13例慢性胰腺炎及15例正常胰腺组织中MK和Ki67蛋白的表达。结果胰腺癌组织中MK和Ki67均高表达,阳性率分别为77.1%(35/49)、81.6%(40/49),二者的表达都与肿瘤组织学分级、临床分期和淋巴结转移有关(P<0.05)。胰腺癌组织中Ki67表达显著高于慢性胰腺炎(3/13,P<0.01)和正常胰腺组织(0/15,P<0.01)。慢性胰腺炎和正常胰腺组织中未见MK阳性表达。胰腺癌组织中MK蛋白的表达与Ki67的表达呈正相关(r=0.4,P<0.05)。结论检测MK蛋白对于胰腺癌的诊断及与慢性胰腺炎的鉴别诊断具有参考价值。MK在胰腺癌中高表达可能与肿瘤的发生发展及细胞增殖密切相关。

Objective To investigate the clinical significance of Midkine （MK） expression in pancreatic carcinoma （PC） and its relationship with proliferation of tumor cells. Methods Immunohistochemical staining method （SP method） was used to study Midkine and Ki67 expression in pancreatic carcinoma samples of 49 cases, chronic pancreatitis （CP） samples of 13 cases and normal pancreatic tissues of 15 cases. Results The expression of MK and Ki67 was high in pancreatic carcinoma tissues, the positive rate being 77. 1%（35/49） and 81. 6o//oo （40/49）, respectively. The expression of MK and Ki67 was significantly correlated with histological grades, clinical stages and lymph node metastasis （P 〈 0. 05）. The expression rate of Ki67 was much higher in pancreatic carcinoma than in chronic pancreatitis （3/13, P 〈 0. 01） and normal pancreatic tissues （0/15, P 〈 0. 01）. The expression of MK was not detected in normal pancreatic and chronic pancreatitis tissues. The expression level of MK had a positive relationship with Ki67 in pancreatic carcinoma （r- 0.4, P 〈 0.05）. Conclusions Determination of MK protein may be used as a useful marker in the diagnosis of pancreatic carcinoma and differential diagnosis between PC and CP. Overexpression of MK in pancreatic cancer may be closely correlated with the development and progression of carcinoma and cell proliferation. MK might be considered as a specific target of gene therapy and drug therapy of pancreatic carcinoma. Specific blocking of MK expression may play a role in inhibiting proliferation of pancreatic cancer cells.