脾虚大鼠脑内催产素受体基因表达变化及归脾汤的影响

Effect of decoction for invigorating the spleen on expression of oxytocin receptor gene in the brain of rats with splenic asthenia

目的:观察脾虚模型脑内对学习记忆有促进作用的催产素受体基因表达变化及归脾汤的影响作用。方法:实验于2002-03/2005-03在北京中医药大学基础医学院完成。清洁级雄性Wistar大鼠30只,体质量(160±10)g,常规适应性饲养1周后,随机抽签法分成正常组、模型组、治疗组,每组10只。采用苦降泻下、饮食失节加劳倦过度法建立脾虚大鼠模型,模型组每日灌胃造模药7.5g/(kg·d);治疗组造模处理加胃饲归脾汤7.5g/(kg·d)。每日上午对除正常组外的2个组胃饲造模药,正常组予等量生理盐水;下午予治疗组胃饲相应中药。连续6周。原位杂交法检测下丘脑腹侧核、海马CA1区、前额叶皮层催产素受体mRNA表达变化。结果:纳入大鼠30只,均进入结果分析。①模型组上述脑区催产素受体mRNA表达阳性反应物明显升高,与正常组比较差异显著(模型组:19.60±3.51,29.62±3.98,20.13±3.22;正常组:8.11±1.23,11.00±1.42,4.94±0.54;P<0.01)。②治疗组上述脑区的催产素受体mRNA表达阳性反应物明显降低,与模型组比较差异显著(治疗组:9.59±1.34,13.00±1.67,5.38±0.96;模型组:19.60±3.51,29.62±3.98,20.13±3.22;P<0.01),与正常组比较无显著差异。结论:脾虚模型学习记忆下降变化,与其脑内对学习记忆有促进作用的催产素受体基因表达改变有关,归脾汤可能通过调节催产素受体基因表达而影响其学习记忆能力。

AIM： To observe the expression＇s change of oxytocin receptor gene that is helpful for learning and memory abilities of brains and the effect of decoction for invigorating the spleen in brains of rat model with splenic asthenia, METHODS： The experiment was carried out in the College of Basic Medicine, Beijing University of Chinese Medicine from March 2002 to March 2005. Thirty cleaning male Wistar rats weighing （160±10） g were adopted and randomly divided into normal group, model group and treatment group, with 10 rats in each, After the rats were regularly fed for one week, splenic asthenia model was established by the methods of catharsis, intemperance of taking food and overstrain, Model group was induced by 7,5 g/kg modeling drugs; while treatment group was injected with 7.5 g/kg decoction for invigorating the spleen, both administrations via intragastric cavity on every morning, whereas equal volume of saline for normal group; on every afternoon, treatment group was given TCM therapy intragastrically. The duration was successively 6 weeks. Hybridization in situ was used to detect the expressions of oxytocin receptor mRNA in ventral nucleus of hypothalamus, hippocampus CA 1 region and prefrontal lobe cortex. RESULTS： Totally 30 rats were involved in the result analysis. ① Compared with normal group, the expressions of oxytocin receptor mRNA were increased obviously in model group （model group： 19.60±3.51, 29.62±3.98, 20.13±3.22; normal group： 8.11±1.23, 11.00±1.42, 4.94±0.54; P 〈 0.01）. ②Compared with model group, the expressions of oxytocin receptor mRNA were declined obviously in treatment group （treatment group： 9.59±1.34, 13.00±1.67, 5.38±0.96; model group： 19.60±3.51, 29.62±3.98, 20.13±3.22; P 〈 0.01）. There was no significant difference between treatment group and normal group. CONCLUSION： The change of oxytocin receptor gene expression may lead to the decrease of learning and memory abilities in rat ,model of splenic asthenia and.decoction for invigorating the spleen can affect the learning and memory abilities by regulating the expressions of oxytocin receptor gene.