腹泻康对脾虚泄泻证大鼠免疫和消化吸收功能的影响

Effect of Fuxiekang pill on the immune,digestive and absorptive functions of rats with splenasthenic diarrhea

目的:通过观察腹泻康对脾虚泄泻证模型大鼠一般状态、免疫、消化吸收功能的影响,探讨其对脾虚泄泻证的防治作用。方法:实验于2006-06/08在军事医学科学院放射与辐射医学研究所药理与毒理研究室完成。健康SD大鼠70只,随机分成7组,即对照组、模型组、柳氮磺胺吡啶0.5g/kg组、葛根芩连丸0.75g/kg组、腹泻康1.6,0.8,0.4g/kg剂量组,每组10只,雌雄各半。造模:按20mL/kg灌服20%番泻叶浸剂,1次/d,连续7d。在灌服3d后,禁食不禁水32h,由肛门(深入8cm)注入80g/L的乙酸0.5mL,捏紧大鼠肛门,倒提20s。造模同时每天在给20%番泻叶浸剂4h后给予相应药物治疗7d,第8天检测大鼠稀便率(稀便粪粒数/总粪粒数);胸腺指数(胸腺质量/体质量)、脾脏指数(脾脏质量/体质量);血清D-木糖含量、淀粉酶活性、琥珀酸脱氢酶比活性。结果:实验大鼠70只均进入结果分析。①大鼠一般症状:给予药物治疗的各组均较对照组精神好转,食欲增强,稀便减少。②大鼠稀便率:给予药物治疗的各组均较模型组稀便率明显降低,其中葛根芩连丸0.75g/kg组、腹泻康1.6g/kg剂量组效果最显著。③大鼠体质量:实验后模型组、柳氮磺胺吡啶0.5g/kg组、葛根芩连丸0.75g/kg组、腹泻康1.6,0.8,0.4g/kg剂量组均较对照组体质量明显下降,且这6组大鼠实验后体质量均较实验前降低。但高剂量组在5,6d和低剂量组在6d与对照组体质量比较差异无显著性。④大鼠胸腺指数、脾脏指数:给予药物治疗的各组均较模型组有不同程度改善。⑤大鼠血清D-木糖含量、淀粉酶活性和琥珀酸脱氢酶比活性:模型组均较对照组明显下降(P<0.01),柳氮磺胺吡啶0.5g/kg组、葛根芩连丸0.75g/kg组和腹泻康1.6,0.8,0.4g/kg剂量组上述指标均有不同程度的改善。结论:腹泻康对脾虚泄泻证大鼠的免疫和消化吸收功能有明显的防治作用,其中腹泻康1.6g/kg剂量组效果最显著。

AIM： To probe into the preventive and therapeutic effect of Fuxiekang capsule on splenasthenic diarrhea by studying its effect on the general symptoms, immune and digestive functions of rat models of splenasthenic diarrhea.METHODS： The experiment was conducted in the Laboratory of Pharmacology ＆ Toxicology, Institute of Radiation Medicine, Academy of Military Medical Sciences between June and August 2006. Seventy healthy SD rats were randomly divided into 7 groups： The control group, model group, 0.5 g/kg sulfasalazine （SS） group, 0.75 g/kgs Gegen Qinlian pill group and 1.6, 0.8 and 0.4 g/kg Fuxiekang groups with 10 rats （half males and half females） in each group. Modeling： Rats were drenched with 20% Folium sennae infusion according to 20 mL/kg once a day for 7 continuous days. Three days after that, rats were banned of the diet but water for 32 hours, and then injected of 0.5 mL of acetic acid （80 g/L） per anum as deep as 8 cm. Rats were clenched of the anus and then kept inversely for 20 s. At the same time of modeling, rats were given corresponding drug treatments at 4 hours after administration of Folium sennae, and the rate of loose stool （times of diarrhea/times of stool） was detected on the 8^th day, and the index of thymus （IT=thymic weight/body mass）, the index of spleen （IS=splenetic weight/body mass）, serum D-xylose content, activity of amylase and specific activity of succinate dehydrogenase were determined simuhaneously. RESULTS：A total of 70 rats were involved in the analysis of results. ① Rats＇ general symptoms： Rats received drug treatments were better than rats in the control group, and their appetite was stimulated with decreased diarrhea. ② Rat＇s rate of loose stool： It was significantly lower in all treatment groups than that in the control group, and the effects in 0.75 g/kg Gegen Qinlian pill group and 1.6 g/kg Fuxiekang group were remarkably better. ③ Rat body mass： The body mass of rats after the experiment was significantly lower in the model group, 0.5 g/kg SS group, 0.75 g/kgs Gegen Qinlian pill group and 1.6, 0.8 and 0.4 g/kg Fuxiekang groups than that in the control group. Besides, the body mass of rats in these 6 groups was significantly decreased after the experiment than that before the experiment. However, the body mass of rats in high-dose group on the 5^th and 6^th days and in low-dose group on the 6th day was not significantly different from that in the control group. ④ The indexes of thymus and spleen of rats： Those in all treatment groups were improved differently than those in the model group. ⑤Serum D-xylose content, activity of amylase and specific activity of suecinate dehydrogenase： Those in the model group were remarkably lower than those in the control group （P〈 0.01）, and those in 0.5 g/kg SS group, 0.75 g/kg Gegen Qinlian pill group and 1.6, 0.8 and 0.4 g/kg Fuxiekang groups were improved to different extent. CONCLUSION： Fuxiekang pill has marked preventive and therapeutic effects on the immune, digestive and absorptive functions of rats with splenasthenic diarrhea, and the effect in 1.6 g/kg Fuxiekang group is remarkably better.