羊睾丸提取液对小鼠受损睾丸支持细胞一氧化氮合酶活性的影响(英文)

Effect of goat testis extract on nitric oxide synthase activity in injured Sertoli cells of mice

背景:大量研究证明,一氧化氮合酶/一氧化氮不仅对维持睾丸支持细胞正常功能起重要作用,而且影响精子的发生、活动及受精能力。目的:观察羊睾丸提取液对重金属铅致损小鼠睾丸支持细胞一氧化氮合酶活性的影响。设计:随机对照动物实验。单位:吉林医药学院组织胚胎学教研室。材料:实验于2004-03/2005-08在吉林医药学院组织胚胎学实验室(解放军总后勤部重点实验室)完成。选用健康昆明种雄性小鼠30只,按随机数字表法分为3组,即对照组、睾丸损伤模型组和羊睾丸提取液组,每组10只。方法:睾丸损伤模型组和羊睾丸提取液组小鼠给予100g/L醋酸铅0.2mL/(只·d),5次/周,连续2周,停药1周。羊睾丸提取液组同时腹部皮下注射羊睾丸提取液0.5mL/(只·d)。对照组给予与实验组溶剂等量的重蒸馏水。染毒期满禁食12h后,称体质量后断头处死,解剖取出双侧睾丸,立即称质量,甲醛固定后切片,应用还原型烟酰胺腺嘌呤二核苷酸磷酸-黄递酶组织化学方法结合显微图像分析,观察各组小鼠睾丸支持细胞的一氧化氮合酶活性变化。主要观察指标:染毒期满后各组小鼠的体质量、双侧睾丸质量及睾丸支持细胞一氧化氮合酶吸光度值。结果:30只小鼠全部进入结果分析,无脱失。①染毒期满后羊睾丸提取液组小鼠的体质量、双侧睾丸质量及一氧化氮合酶A值均显著高于睾丸损伤模型组[(22.47±3.49)g,(0.113±0.021)g,0.236±0.020;(19.13±3.46)g,(0.089±0.017)g,0.146±0.023(t=2.151～3.314,P<0.05～0.01)]。②睾丸损伤模型组睾丸一氧化氮合酶阳性支持细胞肿胀变性;羊睾丸提取液组的一氧化氮合酶阳性支持细胞形态接近对照组。结论:羊睾丸提取液可以提高重金属铅致损小鼠睾丸支持细胞的一氧化氮合酶活性,对睾丸损伤有一定的修复和保护作用,可为临床治疗男性不育提供新思路。

BACKGROUND： Considerable studies demonstrate that nitric oxide synthase（NOS）/nitrie oxide （NO）plays an important role in maintaining normal function of Sertoli cells, and influences spermatic generation and activation as well as fertilizability. OBJECTIVE： To Observe the effect of goat testis extract on NOS activity in Sertoli cells of mice with testis injury caused by heavy mental Pb. DESIGN： Randomized controlled animal trial. SETTING： Department of Histology and Embryology, Jilin Medical College. MATERIALS： This trial was carried out in the laboratory of Histology and Embryology, Jilin Medical College （Key laboratory of the general logistics department of P.L.A） during March 2004 to August 2005. Thirty healthy Kunming male mice were involved and randomized into 3 groups, with 10 in each group： control group, testis injury model group （model group） and goat testis extract-treated group （treatment group）. METHODS： The mice in the model group and experimental group were daily administrated with 100 g/L lead acetate, 0.2 mL/（mouse·d）, 5 times/wk within 2 weeks, then withdrawal for 1 week. Simultaneously, the mice in the treatment group were subcutaneously injected with goat testis extract at 0.5 mL/（mouse·d）. The mice in the control group were given redistilled water of the same dose as that in the treatment group. After being poisoned fully, the mice were fasted for 12 hours and weighted, finally sacrificed by decapitation. Bilateral testis were dissected, immediately weighted, fixed with formalin and sliced. The NOS changes in Sertoli cells of mice in each group were observed with reduced nicotinamide-adenine dinucleotide phophate-diaphorase（NADPH-d） histochemical method combined with microscope image. MAIN OUTCOME MEASURES： Body mass, bilateral testis mass and NOS absorbance （A） in Sertoli cells of mice in each group after contamination expiration. RESULTS： All the 30 mice were involved in the result analysis, without deletion.①After contamination expiration, the body mass, bilateral testis mass and NOS A value in treatment group were significantly than those in the model group [（22.47±3.49） g vs. （19.13±3.46） g;（0.113±0.021 ） g vs. （0.089±0.017） g; 0.236±0.020 vs. 0.146±0.023, t=2.151-3.314,P〈0.05-0.01]; ② In the model group, NOS positive Sertoli cells swelled and degenerated; The morphology of NOS positive Sertoli cells in the treatment group was close to that in the control group. CONCLUSION： Goat testis extract can boost the NOS activity in Sertoli cells of mice with testis injury caused by heavy mental pliumbum and has some repairing and protective effect on testis injury, which can provide new thinking for treatment of male sterility.