摘要
目的:HPLC测定复方泰痹胶囊Ⅰ号中大黄素含量。方法:以AlltimaC18柱,甲醇-水-磷酸(80:20:0.04)为流动相:流速:1.0mL/min。检测波长:347nm。结果:大黄素线性范围:5—50μg/mL,r=0.9993,平均回收率为98.3%,RSD=2.67%(n=5)。结论:方法倘使、准确、重现性好,可作为该制剂的含量测定方法。
Objective :To stablish the determination method of emodin tn Compound Taibi Capsules - Ⅰ with HPLC. Methods : AlltimaC18 columm was used. The mobile phase was methanol -water- phosphoric acid( 80: 20: 0.04)with 1.0mL/min of flow rate, and the detection wavelength was at 347nm. Results: The linear rang of emodin concentration was from 5 to 50 mg/mL and correlation coeffillient was 0. 9993. The average recovery of sample was 98.3% and RSD = 2.67% ( n = 5 ). Conclusion : The method is simple, accurate and reproducible. It can be used for determination of emodin Compound in Taibi Capsules - Ⅰ .
出处
《中医药学刊》
2006年第12期2239-2240,共2页
Study Journal of Traditional Chinese Medicine
基金
广西自然基金资助项目(0236057)