抗纤软肝颗粒药物血清对肝星状细胞整合素信号及凋亡的影响

Effects of Kangxian Ruangan Granule(KXRG) on the Expression of FAK mRNA in Hepatic Stellate Cells

目的:探讨抗纤软肝颗粒药物血清对肝星状细胞增殖、凋亡的影响及整合素信号通路的下游信号分子FAK在其中的作用。方法:将传代培养的大鼠肝星状细胞系(HSC-T6)与中药复方抗纤软肝颗粒药物血清(5%、10%、20%)共同培养48h后,应用MTT法测定细胞增殖;应用流式细胞仪测定细胞凋亡;采用逆转录-聚合酶链反应(RT-PCR)方法检测FAK mRNA的表达。结果:抗纤软肝颗粒药物血清,均能显著抑制HSC-T6增殖,20%药物血清组作用最强(P<0.05);流式细胞仪测定结果显示抗纤软肝颗粒药物血清均可诱导HSC凋亡(P<0.05);抗纤软肝颗粒药物血清能够使HSC的FAK mRNA的表达下调。结论:抗纤软肝颗粒能够抑制HSC增殖及诱导凋亡HSC。其作用可能与其抑制整合素信号通路下游信号分子FAK mRNA的表达有关。

Objective：To investigate the Effects of KXRG on the expression of FAK mRNA in Hepatic Stellate Cells. Methods： The rat hepatic stellate cells line HSC - T6 were incubated with various concentrations of KXRG medicated sera （5%, 10 % ,20% ） for 48h. Ttle cell proliferation was measured by MTT assay. The cell apoptosis was analyzed by flow cytometry, the expression of focal adhesion kinase （FAK） mRNA in HSC was detected using RT-PCR. Results： medicated sera of KXRG could markedly inhibit the proliferation of HSC. With in the range of the safe concentration, the effect of the group of 20% medicated sera was strongest （P 〈 0.05）. In apoptosis medicated sera of KXRG could induce apoptosis （P 〈 0.05） , the expression of FAK mRNA was down - regulated. Conclusion ： KXRG could down regulate the expression of FAK mRNA to inhibit HSC proliferation and induced HSC apoptosis.