益肾调督针法对实验大鼠脑梗死恢复期TNF-α、IL-6和IL-8的影响

The Influence of Kidney-reinforcing and Du Meridian-regulating Acupuncture on TNF-α,IL-6 and IL-8 in Rats in the Convalescent Stage of Cerebral Infarction

目的观察益肾调督针法对脑梗死恢复期治疗及预防再梗的作用机制。方法将实验大鼠随机分为对照组、模型组、益肾调督针法组、常规针法组和非穴针刺组各10例,用血栓栓塞法制备局灶性脑梗死模型,观察治疗前后大鼠神经功能改变,应用双抗体夹心ELISA法分别测定各组大鼠血清中TNFα-、IL-6和IL-8的含量。结果治疗前后大鼠神经功能评分以益肾调督针法组及常规针法组有效,差异具有显著性(P<0.01);模型组大鼠血清中TNFα-、IL-6和IL-8含量较对照组明显升高(P<0.01),施予针刺治疗后各针法组含量均有不同程度的下降。结论益肾调督针法可以有效地抑制TNFα-、IL-6和IL-8的表达,从而在脑梗死恢复期治疗及预防再梗方面发挥作用。

Objective To investigate the mechanisms of kldney-reinforcing and Du Meridian-regulating acupuncture treatment and prevention of re-infarction during the convalescent stage of cerebral infarction. Methods Experimental rats were randomly allocated to control, kidney-reinforcing and Du Meridian-regulating acupuncture, conventional acupuncture and non-point acupuncture groups, 10 each. A model of cerebral local infarction was made by blood clot embolism. A change in rat＇ s neural function was evaluated after treatment. Serum TNF-α, IL-6 and IL-8 contents were measured by double antibody-sandwich enzyme linked immunosorbent assay （ELISA） in each group of rats. Results A comparison of rats＇ neural function scores between pretreatment and posttreatment showed efficacy in the kidney-reinforcing and Du Meridian-regulating acupuncture and conventional acupuncture group; there was a significant difference （P 〈0.01 ）. Rat＇s serum TNF-α, IL-6 and IL-8 contents were significantly higher in the model group than in the control group （P 〈0.01 ） and decreased in different degrees in various acupuncture groups after treatment. Conclusion Kidney-reinforcing and Du Meridian-regulating acupuncture can effectively inhibit the expressions of TNF-α, IL-6 and IL-8 and thereby play a role in treatment and prevention of re-infarction during the convalescent stage of cerebral infarction.