接种TMV的烟草高温胁迫后防御酶活性的变化

Changes of Defense-related Enzyme Activities in TMV-inoculated Tobacco Following High Temperature Stress

采用温度转换和人工接种试验研究了接种TMV的烟草经高温胁迫后防御酶活性的变化。结果表明:烟草接种TMV后经过高温处理(48℃,24h),防御酶中的超氧化物歧化酶(SOD)活性变化较小;过氧化氢酶(CAT)活性在处理后2d内没有明显变化,2d时高于对照;过氧化物酶(POD)、苯丙氨酸解氨酶(PAL)活性均高于对照;多酚氧化酶(PPO)活性在大部分阶段高于对照。因此,CAT、POD、PPO、PAL活性的变化可能与高温诱发烟草对TMV的抗性有关。

By means of temperature shift and artificial inoculation, the change of defense-related enzyme activity in TMV-inoeulated tobacco following high temperature stress was determined. The results showed that in TMV-inoculated and high temperature treated （48℃, 24h） tobacco, the activity of superoxide dismutase （SOD） changed a little; catalase （CAT） activity had no obvious change until 48 hours after treating, it began to decrease from the third day and increased slightly on the fifth day; the activities of peroxidase （POD） and phenylalanine ammonialyase （PAl,） were higher than those in the check; polyphenoloxidase （PPO） activity was higher than that in the check in most stages. Therefore, there is a possible relationship between the change of the activity of CAT, POD, PPO, PAL and the TMV resistance of tobacco induced by high temperature.