纤维化淤胆性肝炎肝组织α-SMA及Ⅰ、Ⅲ型胶原的表达

Expressions of α-smooth muscle actin and collagen type Ⅰ,Ⅲ in biopsy liver tissue from patients with fibrosing cholestatic hepatitis

目的了解纤维化淤胆性肝炎(FCH)肝星状细胞(HSC)活化状态及其与肝纤维化的关系。方法肾移植术后乙型肝炎病毒(HBV)相关性FCH患者9例,正常者5例作为对照。用免疫组织化学的方法检测肝组织α-平滑肌肌动蛋白(α-SMA)及Ⅰ、Ⅲ型胶原蛋白的表达。结果FCH肝组织α-SMA与Ⅰ、Ⅲ型胶原蛋白表达显著增加,表达分布部位一致,定量分析呈明显正相关(P<0.01)。结论肾移植术后HBV相关性FCH患者肝纤维化的发生与HSC的活化状态有关。

Objective To observe expressions of α - smooth muscle actin（α - SMA） and collagen type Ⅰ ,Ⅲ deposition in the liver tissues, and to evaluate potential mechanism of fibrosis in patients with fibrosing cholestatic hepatitis （FCH） by collagen synthesis. Method Immunohistochemical staining was used to detect the expressions of α - SMA and contents of type Ⅰ ,Ⅲ collagen proteins, which were from the specimens of the biopsy liver in 9 cases with FCH associated with hepatitis virus B （HBV） developed following renal transplantation and in 5 cases without liver disease（ control group） , respectively. Results The expressions of α - SMA and type Ⅰ , Ⅲ collagen proteins in the patients with FCH were significantly higher than those in the control group. The expression and distribution pattern of α - SMA and type Ⅰ , Ⅲ collagen proteins were almost identical, and quantitative analysis showed a positive correlation （ P 〈 0. 01 ）. Condusion The state of hepatic stellate cell （HSC） activation may contribute to hepatic fibrosis in the patients with FCH.