牛奶中替米考星残留量的检测方法研究

Determination of Tilmicosin Residues in Milk by HPLC

建立了牛奶中替米考星残留量的检测方法。样品用乙腈提取，C18柱净化，在290nm波长下进行HPLC测定。在空白牛奶中添加0．025、0．05、0．10μg/mL浓度的替米考星时，该方法的平均回收率在72．1％～85．8％之间，日内变异系数在7．0％～10．7％之间，日间变异系数在3.5％～5．4％之间，检测限为0．005μg／mL。该方法适用于牛奶中替米考星残留量的测定。

A method for determination of tilmicosin residues in milk was developed. The procedure involved extracting tilmicosin from milk samples with acetonitrile, using a C 18 cartridge of solid-phase extraction for clean-up of the extract. The extract was concentrated and analyzed by HPLC with UV detection at 290 nm. The method was validated with control milk fortified tilmicosin at 0. 025,0.05,0.10 μg/mL. The average recoveries ranged from 72.1% to 85.8%, with intra-day coefficients of variation were in the range from 1. 0% to 10.7% , and inter-day coefficients of variation were in the range from 3.5% to 5.4%. The limit of detection was 0. 005 μg/mL. The method is suitable for use to determinate tilmicosin residues in milk.