脑心通胶囊对不稳定斑块兔TLR-2和TLR-4炎性信号转导因子的影响

Effect of Naoxintong Capsule on TLR-2 and TLR-4 Signal Transducer Element in Rabbits with Vulnerable Plaque

目的探讨脑心通胶囊稳定易损斑块的机制。方法45只兔给予球囊损伤腹主动脉及高脂饲料喂养,12周末造模兔随机分为自然消退组、脑心通组和辛伐他汀组,每组15只。24周末在腹主动脉斑块处转染携带人野生型p53基因,2周后给予中国斑点蝰蛇毒(CRVV)和组胺触发斑块破裂。于26周末通过免疫组化、WesternBlotting及实时定量RT-PCR分析,检测用药后经p53基因转染处Toll样受体2(TLR-2)、Toll样受体4(TLR-4)和核转录因子κB(NF-κB)的表达。结果转染后动脉粥样硬化斑块处有大量TLR-2、TLR-4和NF-κB表达,尤其集中于内皮细胞和巨噬细胞及炎性细胞浸润处。三者在脑心通组和辛伐他汀组的表达较自然消退组显著降低。结论TLR-2、TLR-4的表达量可预测动脉粥样硬化(AS)斑块的进展,脑心通胶囊可通过降低TLR-2、TLR-4的过度表达而稳定斑块。

Objective To study the effect of Naoxintong Capsule on vulnerable atherosclerotic plaques in rabbit model and to investigate the possible molecular mechanism. Methods Forty - five rabbits were underwent balloon induced abdominal aortic wall injury and then fed with a diet of 1% cholesterol. At the end of 12th week, rabbits were divided randomly into regression group, Naoxintong group and simvastatin group. At the end of 24th week recombinant adenovirus carrying human wild type p53 gene were injected into the aortic segments rich in plaques. Two weeks later, the rabbits underwent pharmacological triggering by Chinese Russell＇s Viper Venom and histamine. At the end of 26th week, toll- like receptor 2 （TLR- 2）, toll - like receptor 4 （TLR 4） and nuclear factor- κB （NF- κB） were examined with western blot analysis and real - time RT- PCR detection. Results The results showed TLR2, TLR4 and NF-κB expression were significantly lower in Naoxintong and simvastatin group than that in control group. Conclusion The plaques could be stabilized by Naoxintong Capsule due to anti - inflammation as simvastatin.