HPLC测定元宝枫中游离和水解没食子酸的含量

Determination of free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC

目的:用HPLC测定元宝枫中游离和水解没食子酸的含量。方法:采用高效液相色谱法在D iamonsil C18色谱柱(5μm,4.6 mm×250 mm),流动相:甲醇-0.025 mol/L磷酸水溶液(15∶85);流速:1.0 mL/m in;检测波长:272 nm;柱温:室温;分离测定了元宝枫叶中游离和水解没食子酸的含量。结果:游离没食子酸含量为0.045%,RSD为1.06%;水解没食子酸含量为1.456%,RSD为2.43%。结论:游离没食子酸含量较少,水解没食子酸含量大,且不同季节含量不同。

AIM ： To detect free and hydrolysable gallic acid in Acer Truncatum Bunge by HPLC. METHODS ： A method for determining gallic acid in leaves of Acer Truncatum Bunge. The operating conditions HPLC were Diamonsil C18 column （5 μm, 4.6 mm × 250 mm） at room temperature, methanol -0.025 mol/L phosphoric acid solution（ 15： 85） as mobile phase at a flow of 1.0 mL/min and UV detection at 272 nm. RESULTS： The contents of free and hydrolysable gallic acid were 0.045% and 1. 546%, repectively. The relative standard deviations of free and hydrolysable gallic acid were 1.06% and 2.43%, respectively. CONCLUSION ： The contents of free and hydrolysable gallic acid in Acer Truncatum Bunge in different periods are different.