菊花二倍体野生种与栽培种间杂种的幼胚拯救

Embryo Rescue of Interspecific Hybrids between Wild Diploid Dendranthema Species and Dendranthema morifolium

对二倍体野生种甘菊、菊花脑、异色菊与栽培菊花进行远缘杂交与杂种幼胚拯救研究。结果表明:影响二倍体野生种与栽培菊花杂种幼胚拯救的关键因素是基因型和幼胚拯救时期,较适宜的时期是授粉后13～18d;基本培养基对幼胚拯救影响不大,较适宜的激素配比为BA2.0mg.L-1+NAA2.0mg.L-1;二倍体野生种作母本与栽培菊花杂交幼胚拯救的成功率明显高于反交。RAPD及形态学鉴定结果证实了杂种的真实性。

Embryo rescue of interspecific hybrids between wild diploid species Dendranthema lavandulifolium, D. nankingense, D. dichrum and cultivated species D. morifolium was studied in this paper. The results indicated that the genotypes and the days of embryo rescue were important factors. 13 - 18 days after pollination were the suitable time for embryo rescue. Medium had little effect on ovary culture. Beneficial proportion of hormones was BA 2.0 mg·L^-1 ＋ NAA 2.0 mg·L^-1 The rescue result was better when wild diploid species used as female parents than as male parents. RAPD and morphological analysis showed that they were real hybrids.