刺槐群体等位酶分析

Allozyme Genetic Investigation on Robinia pseudoacacia

Using 608 Robinia pseudoacacia materials gathered from 13 provinces in China,7 allozyme were electrophoreticlly analyzed in order to detect the genetic diversity of Robinia pseudoacacia population in China.The desirable 7 allozym systems were Amy,Fe,Lap,Idh,Mdh,6-Pgd,Skd.The Ae ranged from 1.592 8～2.761 2.The Ho ranged from 0.187 6～ 0.676 6.The F varied from-0.151 2～0.496 8.The process of making starch gel,model of electrophoretic zymograms and the experience in describing the locus and allele were discussed in detail in this article.As well as the Robinia pseudoacacia population in America and Europe,the Robinia pseudoacacia genetic diversity in China is also high.The results indicated that new Robinia pseudoacacia ecotypes were formed in China under different ecological environment,also,it seemed impossible for gene drift occur in Robinia pseudoacacia population in China.Compared with other genetic markers,allozyme marker still has its unique advantages in detecting population genetic diversity.