肝素酶mRNA在乳腺癌组织中的表达研究

Study of the expression of heparanase mRNA in human breast cancer

目的探讨肝素酶在乳腺癌中的表达和意义。方法应用原位杂交技术检测乳腺癌组织中肝素酶mRNA的表达情况、结合微血管密度、采用SPSS11.5统计软件对病人的临床病理特征等进行分析。结果80例乳腺癌中肝素酶阳性表达53例(66.25%),10例乳腺良性肿瘤阳性表达仅有1例(10%)。乳腺癌中肝素酶的表达明显高于乳腺良性肿瘤(P<0.05)。同时对临床病理特征分析发现肝素酶与临床分期、病理分级、淋巴结转移和肿瘤大小有关(P均<0.05)。结论肝素酶与乳腺癌的血管生成和浸润转移有关;肝素酶可以作为判断乳腺癌浸润转移的重要指标。

Objective To investigate correlation of heparanase （Hpa） expression to angiogenesis and prognosis of breast cancer. Methods The Hpa mRNA in the tissue of breast cancer were detected with In situ hybridization and immunohistochemical technique. Patients＂ clinical and pathological characteristics were analyzed with SPSS11.5. Results Fifty - three （66, 25% ） of 80 expressed Hpa mRNA in breast cancer; in contrast, only 1 （10%） of 10 expressed Hpa mRNA in benign breast tumor. The expression of Hpa mRNA in breast cancer was significantly higher than that of benign breast tumor （P 〈 0.05 ）. Hpa expression in the breast carcinoma tissue was associated with clinical stage, pathological grade, tumor size,and lymph node metastases （ P 〈 0.05 ）. Clonclusion Hpa is associated with the angiogenic and the invasiveness and metastasis of the breast carcinoma. It can be used as an important marker for the invasiveness and metastasis of the breast carcinoma.