蒙古黄芪组织培养及同源四倍体的诱导与鉴定

Studies on Tissue Culture of Astragalus Membranaceus Bge

通过蒙古黄芪组织培养技术的正交试验,确定了蒙古黄芪最佳繁殖培养基:MS+BA0.6 mg/L+IAA0.2 mg/L+PP3330.2 mg/L;叶作为诱导愈伤组织的外植体优于茎,最适培养基:MS+BA2.0 mg/L+KT1.0 mg/L+NAA0.5 mg/L;最佳生根培养基:1/2MS+IAA 0.5 mg/L+IBA2.0 mg/L+ABT2.0 mg/L。建立了蒙古黄芪快速繁殖体系;在无菌条件下利用秋水仙碱诱导蒙古黄芪,获得62个染色体加倍遗传稳定的变异试管苗株系,通过试管苗根尖染色体鉴定,对诱导获得的蒙古黄芪染色体加倍株系进行初步筛选,为进一步获得蒙古黄芪优良品系奠定基础。

The fittest media for rapid-propagation of Astragalus membranaceus was established by orthogonal test in tissue culture process. The results indicated that the strong green clump-buds could be induced on the MS media containing BA 0.6mg/L, IAA 0.2 mg/L and PP333 0.2 mg/L. I.eaves were the best explants of inducing callus on the MS media containing BA 2.0 mg/L, KT 1.0 mg/L, NAA 0.5 mg/L. The strong root was induced on the 1/2MS media containing IAA 0.5 mg/L, IBA 2.0 mg/L and ABT 2.0 mg/L. The immersing buds in colchine solution, and 62 lines of autotetraploid plants were obtained for further selection and breeding into new varieties for commercial production.