影响Ca^(2+)代谢和钙通道的药物对小麦受叶锈菌侵染后诱发的HR的作用

The role of drugs affecting calcium metabolism and calcium channel on HR induced by wheat leaf rust fungus

为进一步探讨小麦抵抗叶锈菌侵染的防卫反应的信号转导机制,利用小麦品种洛夫林10和不同叶锈菌生理小种组成亲和与不亲和组合,研究影响Ca2+代谢和钙通道的药物对小麦受叶锈菌侵染后诱发的HR的作用。结果表明:在接种前给小麦叶片分别预注射Ca2+螯合剂(EGTA)和Ca2+通道抑制剂(LaCl3和Verapamil),均可使寄主叶片发生过敏性反应(HR)的面积明显减少,并且随着注射药物浓度的增高,寄主细胞发生HR的面积逐渐减小;在只注射Ca2+释放剂(A23187)而不接种的试验中,寄主细胞也发生HR,且HR面积随A23187浓度增加而有所增加,但并不成正比。证明胞内Ca2+浓度升高可能是诱发小麦叶片发生HR所必需的,Ca2+在小麦抵抗叶锈菌侵染的信号传导途径中可能发挥重要作用。

In order to discuss the signal transduction mechanism, wheat cultivar Lovrin10 and different leaf rust fungus races were used to form compatible and incompatible interaction groups to study the role of drugs affecting calcium metabolism and calcium channel on HR induced by wheat leaf rust fungus. The results showed that calcium chelator （EGTA） and calcium channel blocker （LaCl3 andVerapamil） were separately injected into wheat leaves before inoculation. Three reagents could obviously reduce the area of dying cells. The higher the concentration, the smaller the area of dying cells; while calcium ionophore A23187 is injected into wheat leaves without inoculation, HR can be detected. The HR area was increased with A23187 concentration, but they were not in direct ratio. These results suggested [Ca^2＋ ] cyt is necessary for wheat to express HR, and calcium may play important role in signal transduction of wheat to resist the infection of leaf rust fungus.