摘要
目的建立大鼠异体肾移植慢性移植肾肾病（CAN）加快模型，为研究非HLA免疫因素及非免疫因索致CAN的病因、病理及病理生理机制提供平台。方法采用雄性Fisher大鼠和Lewis大鼠分别作为供一受体，并以假手术组作为对照，进行异体原位肾移植。受体移植术前对供肾进行强化冷缺血处理1h，于术后4w，8w，12w分别观察各受体血肌酐和移植肾组织病理变化情况。结果移植术后4周Fisher-Lewis组开始出现血肌酐的升高及移植肾CAN的病理改变，8～12周病变渐趋明显，与对照组比较有统计学差异（P〈0．05）。结论以Fisher和Lewis近交系大鼠作为供一受体建立的大鼠异体肾移植CAN加快模型是一种可靠、实用和研究价值高的肾移植实验动物模型。
Objective To set up a chronic allograft nephropathy （CAN） model in rat for the studying in chronic rejection caused by non-HLA and non-immunologic factors. Methods We used Fisher rats as donors and Lewis rats as recipients with modified Kamada＇s surgical procedures. Before recipient＇s operation, the donor kidney was performed with intensive ischemia/reperfusion injury. The recipients were given cy closporine after operation to prevent acute rejection. Results In the 4th , 8th and 12^th weeks after transplantation,the recipientg serum creatinine levels significantly increased than the control group （P〈0.05 or P〈 0. 001）. And the transplanted kidneys gradually showed obviously pathological characteristics of CAN dur ing the period. Conclusion This model is a reliable, valued and useful platform in studying chronic allograft nephropathy of renal transplantation.
出处
《四川解剖学杂志》
2006年第4期15-17,共3页
Sichuan Journal of Anatomy
关键词
肾移植
大鼠
慢性排斥
模型
kidney transplantation
rat
chronic rejection
model
