人参皂甙Rg3对子宫内膜异位症大鼠血管生成的影响

Anti-angiogenesis effect of ginsenoside Rg3 on endometriosis models of rats

目的探讨人参皂甙Rg3对大鼠子宫内膜异位症(EMs)模型,异位组织中血管内皮生成因子(VEGF)和微血管密度(MVD)的影响。方法建立Wistar大鼠子宫内膜异位症模型,3周后将大鼠随机分为空白对照、人参皂甙Rg35mg/kg、10mg/kg和孕三烯酮4组,相应处理8周后,观察各组异位子宫内膜体积和形态学的变化,免疫组织化学法测定血管内皮生长因子(VEGF)在异位内膜的表达,并通过CD31标记异位子宫内膜血管,测定其微血管密度(MVD)。结果(1)治疗后,各给药组移植物呈现不同程度萎缩(P<0.05),其中以Rg310mg/kg组最为明显;(2)各给药组VEGF表达、MVD数量均低于对照组(P<0.05),其中以Rg310mg/kg组最为明显。结论Rg3可以通过调节VEGF表达和MVD数量来抑制EMs大鼠模型异位组织的血管生成。

Objective： To observe the effect of ginsenoside Rg3 on vascular endothelial growth factor（VEGF） and micro-vessel density（MVD） in the ectopic endometrial grafts of endometriosis rat models. Methods： The endometriosis model was established in Wistar rats and the model rats were divided into 4 groups that were treated with normal saline （control）, ginsenoside Rg3 5 mg/kg, ginsenoside Rg3 10 mg/kg and gestrinone, respectively. After 8 weeks of treatment, ectopic endometrium grafts were examined for the volume and histomjorphology. The expression of VEGF was observed by immunohistochemistry（SABC technique） and MVD was determined by immunostaining for CD31. Results：（1） The volume of grafts in all treatment groups was significantly reduced in comparison with that in the control group（P〈0. 05）, with the most significant reduction in Rg3 10 mg/kg group. （2） The expressions of VEGF and MVD in the treatment groups were significantly lower than those in control group（P^0. 05）, with the lowest in Rg3 10 mg/kg group. Conclusion： Ginsenoside Rg3 has an anti-angiogenesis effect on the ectopic endometrial grafts of endometriosis rat models by inhibiting VEGF and MVD.