β-Catenin,cyclinD1及c-myc在肝细胞癌中的表达

Expression of Beta-catenin,cyclinD1 and C-myc Genes in Hepatocellular Carcinoma

目的探讨β-Catenin、cyclinD1及c-myc蛋白的表达与肝癌临床病理参数的关系。方法采用EnVisionTMplus免疫组织化学方法研究β-Catenin、cyclinD1及c-myc蛋白在52例HCC及癌旁肝组织中的表达情况。结果52例HCC中β-Catenin、cyclinD1及c-myc蛋白染色阳性率分别为55.8%、48.1%及53.8%,癌旁肝组织的阳性率为36.5%、25.0%及32.7%,β-Catenin、cyclinD1及c-myc在HCC及癌旁肝组织两者间有显着性差异(P<0.05)。在HCC中β-Catenin的阳性表达与cyclinD1、c-myc的阳性表达密切相关(P=0.0108,r=0.3920;P=0.0295,r=0.3406)且呈正相关;β-Catenin、cyclinD1及c-myc在人肝癌组织中的检出率与肝外转移、术后复发及肿瘤分化程度明显有关(P<0.05),cyclinD1的检出率与门静脉癌栓也明显有关(P<0.05),β-Catenin的检出率与临床分期和门静脉癌栓也有显著性差异(P<0.05)。结论β-Catenin、cyclinD1及c-myc蛋白的过表达可促使肝癌细胞增殖,使肝癌细胞具有更强的侵袭力,与肝癌的发生发展关系密切。

Objective To investigate the correlation between the clinic pathologic parameters of hepatocellular carcinoma（HCC） and the expression of beta-catenin.cyclinD1 and c-myc genes. Methods Immunohistochemical technique（EnVisionTM plus） was applied to detect the expression of beta-catenin, cyclinD1 and c-myc proteins in 52 HCC tissues and surrounding tissues. Results The rates of positive immunosraining of beta catenin, cyclin-D1 and c-myc in the HCC tissues were 55.8%.48. 1% and 53.8%. The rates of positive in the surrounding tissues were 36. 5% ,25. 0% and 32. 7%. The positive rate of betacatenin, cyclinD1 and c-myc was significantly higher in the HCC tissue than in the surrounding tissues （P 〈0. 05）. There was a positive correlation between the beta-catenin expression and cyclinD1 and c-myc in the HCC tissue（P = 0. 0108, r = 0. 3920; P = 0. 0295, r = 0. 3406）. The positive rate of beta catenin, cyclinD1 and c-myc in the HCC tissue was significantly correlated with the presence of extrahepatic metastasis, the recurrence of tumor and the differentiation of tumor（P〈0. 05）. The positive rate of cyclinD1 was significantly correlated with the ortal vein tumor thrombus（P〈0. 05）. The positive rate of betacatenin was significantly correlated with the ortal vein tumor thrombus and the clinical stage （P〈0. 05）. Conclusion The over-expression of beta catenin,cyclinD1 and c-myc proteins may contribute to the proliferation of hepatocellular carcinoma, making them more aggressive and relates to the initiation and progression of HCC.