切割海马伞大鼠海马中65kD和83kD差异蛋白诱导神经干细胞迁移和向神经元分化的作用

Effects of 65 kD and 83 kD differential protein in rats'hippocampi with fimbria transection on inducing neural stem cells migration and differentiation into neurons

目的：探讨切割海马伞大鼠海马中65 kD、83 kD差异蛋白是否具有诱导神经干细胞迁移和向神经元分化的作用。方法：切割海马伞后的海马进行非变性聚丙烯酰胺凝胶电泳,将65 kD、83 kD差异蛋白胶条与大鼠神经干细胞球共培养,计数神经球朝胶条方向1/4扇区内迁移出的细胞数。10 d后行微管相关蛋白2(MAP-2)免疫荧光,计数其中MAP-2阳性神经元数,图像处理其胞体面积和细胞周长。结果：83 kD切割组朝胶条方向迁移的细胞最多,迁移速度最快。其分化的神经元数量多,胞体大、周长长,明显地优于83 kD正常组、65 kD切割组、65 kD正常组和空白对照组。结论：大鼠海马组织中83 kD蛋白具有诱导神经干细胞迁移和向神经元分化的作用。

Objective： To explore the effects of the 65 kD and 83 kD proteins in hippocampi of rats on inducing neural stem cells （NSCs） migration and differentiation into neurons. Methods： Native-PAGE was carried out in the hippocampi with the fimbria transection. The NSCs were cocultured with 65 KD and 83 KI） differential protein gel straps. The number of cells in 1/4 sector towards the gel migrating out of the sphere was calculated. On the 10th day after coculture, microtubule associate protein Ⅱ （MAP-2） irnmunofluorescence was used to detect the differentiation of NSCs into neurons. The number, dimension and perimeter of the MAP-2 positive neurons in 1/4 sector were analyzed with image analysis system. Results： The NSCs in 83 kD transection group migrating towards the gel strap were the most and the migration speed was also the fastest. The MAP-2 positive neurons were the most, the dimension was the biggest and perimeter was the longest in 83 kD transection group. The results of the 83 kD transection group were superior to those of the 83 kD normal group, 65 kD transection group, 65 kD normal group and control group. Conclusion： The 83 kD protein in hippocampi of rats can induce NSCs migrating and differentiating into neurons.