不同年龄大鼠免疫器官的衰老细胞

Senescent cells in immune organs of rats at various ages

目的：研究不同年龄大鼠免疫器官的衰老细胞。方法：应用衰老相关β-半乳糖苷酶组织化学染色显示不同年龄大鼠胸腺、脾、淋巴结的衰老细胞。结果：胸腺内衰老细胞多分布于胸腺小叶皮质和髓质交界处；脾内衰老细胞主要分布于边缘区及脾索；淋巴结内衰老细胞主要分布于皮质淋巴小结之间和髓质。随着年龄的增长胸腺、脾和淋巴结内的衰老细胞逐渐增多。结论：免疫器官胸腺、脾和淋巴结内存在衰老细胞,并随年龄的增长而增多,提示细胞衰老与个体衰老有关。

Objective： To study the senescent cells in the immune organs of the rats at various ages. Methods： The histochemical staining of senescence associated β -galactosidase （SA-β-Gal） was used to study the senescent cells in the thymus, spleen and lymph node of the rats at various ages. Results： The senescent cells of the thymus were mainly distributed in the aera of corticomedullary junction. The senescent cells of the spleen were localized mostly in the marginal zone and the splenic cord. In the lymph node, the senescent cells mainly appeared in the zone between lymph nodules, and in the medullary cord and sinus. The number of the senescent cells in the thymus, spleen and lymph node increased with aging. Conclusion： The cellular senescence occures in the thymus, spleen and lymph node, and the number of the the senescent cells increase with aging. Our results indicate that the cellular senescence may be correlated to the systemic aging.