MK-801对新生大鼠脑外伤后神经元凋亡的影响

Effect of MK-801 on neuronal apoptosis after traumatic brain injury in neonatal rats

目的：探讨N-甲基-D-天冬氨酸(NMDA)受体拮抗剂MK-801对新生大鼠创伤性脑外伤(traumatic brain injury,TBI)后神经元凋亡的影响。方法：建立新生7 d大鼠顶叶皮质挫伤模型,在TBI前30 min、TBI后即刻、TBI后30 min分别给予腹腔注射MK-8011 mg/kg,在TBI后24 h取脑,连续切片,行H-E染色和Caspase-3免疫组化染色,检测脑神经元细胞的损伤和凋亡。结果：MK-801三组不同时间用药组与TBI组相比,在创伤同侧的扣带皮质、顶叶皮质和丘脑神经元凋亡细胞数减少,有显著性差异。其中TBI后即刻用MK-801治疗效果最好。结论：MK-80l能明显减少TBI后神经元的凋亡。

Objective： To study the effect of MK-801, the antagonist of NMDA receptor, on neuronal apoptosis after traumatic brain injury （TBI） in neonatal rats. Methods： Seven-day old rat pups were subjected to concu.ssion trauma on the parietal cortex and were injected with 1 mg/kg MK-801 30 minutes before TBI, immediately after TBI and 30 minutes after TBI. The pups were killed 24 h after injection. The neuronal apoptosis of the brain was investigated by both HE and Caspase-3 immunostaining. Results： Compared with TBI group, the number of apoptosis cells in MK-801-treated group was significantly decreased in the ipsilateral cingulate, parietal cortex and thalamus. The outcome was best when MK 801 was used immediately after head trauma. Conclusion： MK-801 can reduce apoptosis of cells in the immature brain after TBI.