大鼠脑胶质瘤伽玛刀治疗后穹隆下器一氧化氮合酶表达的变化

Expression changes of NOS in subfornicai organ of rats with brain glioma after Gamma Knife radiosurgery

目的：探讨穹隆下器(subfornical organ,SFO)在脑肿瘤免疫过程中的作用及意义。方法：脑胶质瘤大鼠分伽玛刀治疗和对照组。通过NADPH-d和iNOS免疫组化染色,观察SFO阳性细胞面密度,并测定大鼠外周血NO的含量。结果：与对照组大鼠比较,治疗组大鼠SFO处阳性数量明显增多,以其腹内侧区为著。阳性细胞排列呈团状或片状,胞质深染,并可见大量浓染的阳性突起,细胞面密度较对照组非常明显增高。治疗组大鼠外周血NO的含量为(12．63±1．16)μmol/L,非常显著高于对照组的(8．33±0．21)μmol/L)。结论：大鼠脑胶质瘤伽玛刀治疗后NOS和iNOS在SFO的表达显著增多,提示SFO可能与放射治疗引起的神经免疫调节有关。

Objective： To observe the expression change of NOS in subfornical organ （SFO） of rats with brain glioma before and after Gamma Knife radiosurgery. Methods： Forty rats were divided into Gamma Knife treatment and control group. NADPH-d and iNOS immunohistochemistry staining was performed to observe the surface density of postive cells, and the NO content in the blood was determined. Results： Compared with the control group, the positive cells were significantly increased in the Gamma Knife treatment group, especially at the ventralis medialis. The positive cells were arranged in mass and lamellar shape with heavily stained cytoplasm and much positive process. The surface density was significantly increased comparing with that in the control group. The NO content in the blood of the treatment group （12. 63±1.16μmol/L） was significantly increased than that in the control group （8. 33±0. 21μmol/L）. Conclousion： After Gamma Knife treatment, the NOS and iNOS expression in SFO is markedly increased, suggesting that SFO may be related to the neuro-immuno-modulation following Gamma Knife irradiation.