野生种马铃薯PHYA基因cDNA的克隆与表达分析

Cloning and Expression Analysis of PHYA cDNA from Wild Potato (Solanum pinnatisectum Dun.)

采用RT-PCR技术从野生种马铃薯(SolanumpinnatisectumDun.)中克隆到1个光敏色素基因PHYA,其cDNA全长为3466bp,含有一个3372bp的完整开放阅读框,编码一条长1123个氨基酸的蛋白,分子量为125kDa,等电点为5.8。该基因编码的蛋白序列与栽培种马铃薯、番茄和烟草基因编码的氨基酸序列同源性分别为96%、94%和91%。半定量RT-PCR分析表明,PHYA在根、茎和芽中的表达量较高;光对PHYA表达的作用在地上部器官中表现为抑制,而在地下部器官中则促进。

The full-length cDNA of PHYA gene was cloned from wild potato （Solanum pinnatisectum Dun.） by RT-PCR. The cDNA was 3 466 bp long and had a open reading frame （ORF） of 3 372 bp, and the ORF encoded 1 123 amino acid residues with a predicted molecular weight of 125 kDa and an isoelectric point of 5.8. The protein sequence comparison showed that the predicted protein had higher identities to those from Solanum tuberosum, Lycopersicon esculenmm and Nicotiana tabacum （96%, 94% and 91%）. Semi-quantitive RT-PCR indicated that the expression of PHYA was different in each organ and higher in roots, stem and shoots. The influence of light on the expression of PHYA depended on the space position of organs and was promotive in the underground organ and was restrained in the aboveground organs.