乙醇对大鼠性功能影响的实验研究

Relationship Between Ethanol Intake and Sexual Function in Rats

目的:探讨乙醇对大鼠性功能的影响。方法:将60只Wistar雄性大鼠随机分为对照组、10%乙醇剂量组、20%乙醇剂量组、30%乙醇剂量组和40%乙醇剂量组,每组12只。分别以生理盐水、10%、20%、30%、40%食用乙醇各2ml给大鼠灌胃,每日1次。3个月后,观察各组大鼠性行为和药物诱发阴茎勃起的变化;用全自动化学发光分析仪测定各组大鼠血清睾酮的含量;以80μg/kg体重的阿朴吗啡大鼠颈部注射诱发阴茎勃起;用化学比色法测定阴茎平滑肌中一氧化氮合酶(NOS)的总量。结果:10%和20%乙醇剂量组大鼠药物诱发阴茎勃起的次数没有受到乙醇影响(P>0.05),但10%乙醇剂量组大鼠的乘骑和插入潜伏期延长,20%乙醇剂量组大鼠除了射精潜伏期外,其余性行为参数也受到抑制(P<0.05)。而30%乙醇剂量组与40%乙醇剂量组大鼠的性行为表现、药物诱发阴茎勃起实验均明显受到抑制(P<0.05)。与对照组相比,实验组血清睾酮含量和阴茎组织中NOS活性均减低(P<0.05)。结论:大鼠长期乙醇摄入后性功能障碍的发生与剂量有关。

Objective ： To investigate the relationship between the intake of ethanol and sexual function of rats. Methods ： Sixty adult male Wistar rats were randomly divided into five groups： the control, 10% , 20% , 30% and 40% ethanol groups, which received. 0.9% sodium chloride, 10% , 20% , 30% and 40% ethanol solutions respectively at a dose of 2 ml through gastric tubes once a day. Three months later, we observed the effects of ethanol on the sexual function of the rats by their sexual behaviors, the number of apomorphine-induced penile erections, and the content of testosterone in the serum and nitric oxide synthase（NOS） in the penis. Results ： Compared with the control group, the number of apomorphine-induced penile erections in the 10% and 20% ethanol groups was not inhibited significantly（P 〉 0.05 ） , but the latent period of mounting and intromission in the 10% ethanol group was prolonged and the sexual behaviors in the 20% ethanol group were inhibited except the latent period of ejaculation. The sexual behaviors and the number of apomorphine-induced penile erections of the 30% and 40% ethanol groups were inhibited significantly（ P 〈 0.05 ）. Testosterone in the serum and NOS activity in the penis of the experimental groups were reduced （ P 〈 0.05）. Conclusion ： An adequate volume of ethanol does nut induce sexual dysfunction in rats, but long term and excessive intake of ethanol may cause penile erectile dysfunction.