脐血IgE、白细胞介素2及5的测定及其临床意义

Determination and significance of cord blood Ig-E,interleukin-2(IL-2 ) and IL-5 levels

目的探讨新生儿脐血免疫球蛋白E(IgE)、白细胞介素2(IL-2)、白细胞介素5(IL-5)水平及其对预测婴幼儿哮喘发生的意义。方法使用美国I MX型化学发光检测仪,采用免疫荧光法测定37例新生儿脐血IgE含量;用酶标仪、双抗体夹心ELISA法测定172例脐血IL-2、IL-5含量。结果①新生儿脐血IgE水平男性高于女性(P<0·05);②脐血IL-2、IL-5水平性别间差异无统计学意义(P>0·05)。③有家族过敏史的13例中IL-2、IL-5水平低于无过敏家族史者,但差异无统计学意义(P>0·05)。结论脐血中IgE、IL-2、IL-5含量极低;性别对IgE有影响,对IL2、、IL-5无明显影响;家族过敏史可能对脐血IgE、IL-2、IL-5水平有影响,尚待继续观察。

Objective To explore the Ig-E, IL-2 and IL-5 levds of eord blood and the significance of forecasting the possibility of asthma in infant and young child. Methods The IgE levels were measured with irnmumofluorescence method. The IL-2 and IL-5 levels was measured with double antibody sandwich ELISA method. Results （1） The male＇ s IgE levels were significant higher than the female＇s （ P 〈0.05）. （2） There was no significant difference of the IL-2 and IL-5 levels between the two genders（ P 〉0.05）. （3） The IL-2 and IL-5 levels in 13 newborns with familial history of hypersensitiveness was lower than the others, but there was no significant difference between them （ P 〉0.05）. Conclusion The Ig-E, IL-2 and IL-5 levels of cord blood are extremely low. There is no significant difference between the two genders of IL-2 and IL-5 levels. The familial history of hypersensitiveness may influence the IgE , IL-2 and IL-5 levels and more research is needed on it.