摘要
建立了丙酮沉淀-高效液相色谱法制取高纯度超氧化物歧化酶(SOD)的新工艺。利用此法制得电泳级的SOD,总活性回收率为86.0%,纯化倍数提高52倍。小规模的生产实验表明该法具有工艺简单、成本低、效率高、生产周期短等优点。
The new technology for preparing highly purified superoxide dismutase（SOD） by acetone precipitation high performance liquid chromatography（HPLC） was established in this work. The SOD of electro- phoretic rank was prepared by the method with total activity recovery of 86.0%, 52 times purification. According to the small scale production, the method was superior to conventional methods with simpler process, lower cost, higher efficiency and shortened operating periods.
出处
《化学与生物工程》
CAS
2006年第12期32-33,共2页
Chemistry & Bioengineering
基金
陕西省科学技术研究发展计划资助项目(2005k06-G9)
关键词
超氧化物歧化酶
高效液相色谱
纯化
superoxide dismutase（SOD）
high performance liquid chromatography （HPLC）
purification
