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植物多肽激素PA1b的纯化鉴定及功能研究 被引量：6

Isolation,Characterization and Biofunction of PA1b
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摘要 通过稀醋酸低温浸提、海藻酸吸附、盐析、SephadexG25凝胶过滤、Sephacel离子交换层析和高效液相色谱等方法,从豌豆种子中分离纯化出PA1b及其多种异构体,并通过质谱和氨基酸测序进行了鉴定,结果表明:PA1b能抗胃蛋白酶(pepsin)水解,其高度的酶稳定性是由于分子内具有胱氨酸结构模体。此外,PA1b能明显延长体外培养的胰岛β细胞的存活时间,并影响胰岛β细胞的功能。 PAlb, a polypeptide hormone, was isolated from pea seed . Earlier reserch linked it to plant growth, development and defense. PAlb and its isoforms had been isolated from pea seeds and characterized by chromatogram, RP-HPLC and MALIDI-TOF-MS techniques in this report. It was reported that PA1b could resist the degradation of some protease in recent research. It was demonstrated that this property was related to its particular structure, cystine knot motif. In addition, it was reported PAlb could significantly prolong the lifetime of rat pancreatic β cells in vitro ,giving a clue to the new biofunction of PAlb.
作者 陈磊 赵燕英 顿新鹏 陈正望 陆婕
机构地区 华中科技大学生命科学与技术学院
出处 《华中农业大学学报》 CAS CSCD 北大核心 2006年第6期586-590,共5页 Journal of Huazhong Agricultural University
基金 国家"863"计划项目(2002AA214061)资助
关键词 PAlb 反相高效液相色谱 胃蛋白酶 胱氨酸结构模体 胰腺Β细胞 PAlb RP-HPLC pepsin cystine knot motif pancreatic β cells
分类号 Q578.3 [生物学—生物化学]
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参考文献11

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同被引文献63

  • 1杜雯,徐平勇,陈正望.豌豆胰岛素基因克隆、表达及融合蛋白纯化[J].华中科技大学学报（自然科学版）,2004,32(9):102-104. 被引量：3
  • 2向明,汤静,储潼,张程亮,邹晓蕾.人工虫草对链脲佐菌素所致小鼠1型糖尿病的降糖作用及机制[J].中国医院药学杂志,2006,26(5):556-559. 被引量：11
  • 3王征,张天宝,朱玉平,谢宗华.急性毒性体外筛选方法的比较[J].卫生研究,2006,35(3):286-288. 被引量：4
  • 4胡志涛,顿新鹏,张铭,朱洪亮,谢丽,吴政星,陈正望,徐涛.植物多肽PA1b打开胰腺β细胞L型钙通道促使钙内流和细胞分泌[J].中国科学（C辑）,2007,37(3):280-286. 被引量：5
  • 5YAMAUCHI F, SATO K, YAMAGISHI T. Isolation and partial characterization of a salt extractable globulin from soybean seeds[J]. Agric Biol Chem, 1984,48(3): 645-650.
  • 6WATANABE Y, BARBASHOV S F, KONATSU S, et al. A peptide that stimulates phosphorylation of the plant insulinbinding protein:isolation, primary structure and cDNA cloning [J]. Eur J Biochem, 1994,224:167-172.
  • 7HIGC-INS T J, CHANDLER P M, RANDALL P J, et al. Gene structure,protein structure,and regulation of the synthesis of a sulfur-rich protein in pea seeds[J]. Biol Chem J, 1986, 261 (24):11124-11130.
  • 8YAMAZAKI T, TAKAOKA M, KATOH E, et al. A possible physiological function and the tertiary structure of a 4-kDa peptide in legumes[J]. Eur J Biochem, 2003,270: 1269-1276.
  • 9HANCOCK K R, EALING P M, WHITE D W. Identification of sulfur-rich proteins which resist rumen degradation and are hydrolysed rapidly by intestinal proteases[J]. Br J Nutr, 1994, 72:855-863.
  • 10SPENCER D, HIGGINS T J V, FREER M, et al. Monitoring the fate of dietary proteins in tureen fluid using gel electrophoresis[J]. Br J Nutr, 1988,60:241-247.

引证文献6

二级引证文献7

华中农业大学学报
2006年 第6期

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