动物组织中抗菌药物残留的微生物学快速筛选法研究

Fast Screening Methods for Antimicrobial Agents Residues in the Edible Tissue

以巨大芽孢杆菌为受试菌,建立一种高度敏感的动物组织中抗菌药物残留快速筛选拭子法。拭子法对猪、鸡组织(肌肉、肝脏)青霉素、红霉素、新霉素、庆大霉素的检测限均低于我国规定的最高残留限量,其准确度、精确度及假阴性率等性能指标均符合筛选要求。本方法与国外同类方法(FAST试剂盒)对6种抗菌药物的最低检测限一致。

A high sensitive method was developed to screen antimicrobial residues in rood animat Ussues which is based on the inhibition by the growth of Bacillus megaterium. The detected limit of this method for penicillin, chlortetracycline, erythromycin, neomycin, gentamycin in the tissues of swine and broiler （muscle,kidney and liver） is lower than or near MRLs established in China. Moreover, accuracy, precision and the false-negative results of this method meet the need of screening method. A comparison was made between the swab tests and the same kind of foreign swab tests, which gave quite similar results to 6 antimicrobial agents. It is concluded that the distinction between them was very low and the swab tests should be a good screening method developed in China for the detection of antimicrobiat drugs residues in swine tissue and urine and should have enormous promise in China.