狂犬病患者血清肿瘤坏死因子(TNF)活性测定及其意义

THE TNF ACTIVITY IN SERUM OF RABIES PATIENTS AND ITS SIGNIFICANCE

采用细胞病变结晶紫染色法测定具有特异诊断依据的16例狂犬病患者血清中TNF活性。结果显示患者组TNF活性指数明显高于对照组（P＜0．01）.表明狂犬病毒感染除其特异性地侵犯神经组织，造成神经功能严重紊乱外，也可通过TNF这一类与组织的炎症及坏死密切相关的细胞因子，造成系统性损害，加重病情，加速狂犬病患者的死亡。用细胞病变结晶紫染色法测定狂犬病患者血清中TNF活性，结果判断客观，容易推广。

The TNF activity in serum of 16 rabies patients confirmed by specific laboratory diag nosis and clinical signs was tested with the method of crystal violet assay for TNF's cytotoxity. The data showed that TNF activity index of patients was markedly higher than that of controls (P<0.01). It means that besides the rabies virus attacking nervous system directly and specifically, rabies virus infection might result in systemic damages of the body by some cytokines responsible for tissue inflammation and necrosis, such as TNF, to make the disease more serious, finally to speed up the deterioration of the case up to death. The method for TNF activity assay is easy to be used, and the results is reliable and reproducible.