利用酵母双杂交系统研究植物与病毒蛋白相互作用的进展

Advances of the Interaction between Proteins of Plant and Virus Using Yeast Two Hybrid Method

在长期进化中,植物形成了抵御病毒等病原微生物侵染的精细防御系统。在病毒侵染、复制和传播过程中,其编码的一些蛋白,如外壳蛋白、运动蛋白、复制酶类等能够与植物基因编码的蛋白发生相互作用。酵母双杂交系统是体外研究蛋白质间相互作用的有利工具,不但可以用于研究已知蛋白质的互作,还可以发现新蛋白,揭示特定蛋白互作网络与作用机制,在植物蛋白与病毒蛋白互作研究中已得到广泛的利用。本文主要综述利用酵母双杂交系统研究植物与病毒蛋白相互作用的国内外进展。

During long time evolution, plants developed elaborate defense pathway and complicated mechanisms against virus and other pathogens. Virus proteins, such as coat protein （CP）, movement protein （MP） and polymerase protein, would interact with host plant proteins during virus infection process. The yeast two hybrid system is a useful method to analyze the interaction between proteins in vitro and applied widely in studying the interaction between proteins of plant and virus. This article mainly presented the advances of the protein interaction of plant and virus by using yeast two hybrid during the past decade.