铝酞菁D及其与黄夹甙或维拉帕米合用对肿瘤细胞的光动力治疗作用

Photodynamic Effect of Aluminum Phthalocyanine D Used Alone or in Combination with Thevetoside or Verapamil on Tumor Cells in vitro

目的研究新型光敏剂铝酞菁Ｄ（ＡｌＰＣ－Ｄ）单用及与黄夹甙或维拉帕米合用对体外培养肿瘤细胞的光动力治疗作用。方法细胞经药物作用２ｈ后，用红光辐照一定时间，继续培养２４ｈ，台盼蓝排染法测定药物对肿瘤细胞的抑制率。结果２．５～１０．０μｇ／ｍｌＡｌＰＣ－Ｄ配以５．４或１０．８Ｊ／ｃｍ２的红光射照，能显著杀伤Ｋ５６２和ＳＧＣ７９０１细胞，并有剂量依赖性。０．００１和０．０１μｇ／ｍｌ黄夹甙或单用无效剂量的维拉帕米（５μｇ／ｍｌ）与ＡｌＰＣ－Ｄ合用，能增强后者对Ｋ５６２细胞的光动力作用。结论ＡｌＰＣ－Ｄ对体外培养的肿瘤细胞具有光动力治疗作用，黄夹甙和维拉帕米能增强其作用。

To study the photodynamic effect of aluminum phthalocyanine D(AlPC-D), which is a new type of photosensitizer,used alone or in combination with thevetoside(TS),or verapamil(Ver)on the tumor cells cultured in vitro.Methos After 2 h treated by drugs,the cells were irradiated for certain time by red light,then cultured for 24 h.Inhibition rate of drugs on tumor cells was determined by trypan blue dye exclusion,Results K562 and SGC-7901 cells were markedly inhibited by 2.5￣10.0μg/ml AlPC-D plus 5.4 or 10.8J/cm2 red light irradiation,with dose dependence.The photodynamic effect of ALPC-D on K562 cells was potentiated by 0.001 and 0.01μg/ml thevetoside or nontoxic doses of Ver. Conclusion AlPC-D has photodynamic effect on tumor cells in vitro and this effect can be enhanced by thevetoside or Ver.