正畸加力后大鼠牙周组织中BMP的分布变化及其意义

Distribution of Bone Morphogenetic Protein (BMP) in Periodontal Tissue of Rats during Orthodontic Tooth Movement

本研究首次应用抗牛骨形成蛋白单克隆抗体（ｂＢＭＰ－ＭｃＡｂ）免疫组织化学染色法，观察加力后的大鼠上第一磨牙牙周组织中ＢＭＰ的分布，并与正常不加力组相比较。结果显示：ＢＭＰ在正常牙周组织中主要分布于牙周韧带中，尤其是牙槽骨和牙骨质表面，并与不同部位的骨改建活动相一致；正时加力后，张力区成骨活跃，ＢＭＰ染色较深（强阳性），压力区染色为阴性，只有活跃的破骨细胞染色较深；已接近成熟的新生牙槽骨及牙骨质则染色较弱。结果表明；ＢＭＰ可以作为骨改建活动的标志之一，在正畸骨改建中起着十分重要的作用。

Our study firstly demonstrated the changed distribution of BMP in periodontic tissues during orthodontic movement of maxillary first molars in rats. BMP was stained with immunohistochemical staining technique (ABC method). Using monoclonal antibody to BMP, we found that the staining density in periodontal tissues varied according to different parts and treatment measures. BMP is mostly located in the periodontium, especially near the alveolar bone or cementum, that is always associated with bone remodeling activity. The distribution of BMP was changed during orthodontic tooth movement. The tension zone was intensly stained, while the pressure zone was poorly stained. The newly formed cementum and alveolar bone was moderatly stained. The result indicated that periodontic ligament cells have the property of osteoblast-likecells and osteogenic activity under the condition of orthodontic tooth movement and as a promoting factor of bone remodel activity. BMP take an important role in orthodontic tooth movement.