胃癌雌激素受体和DNA含量检测及其意义

Detection of ER and DNA Content in Gastric Cancer and Its Clinical Significance

应用改进的亲和组化法检测５９例胃癌雌激素受体状况，流式细胞仪检测其中５６例ＤＮＡ含量。结果显示：５９例胃癌ＥＲ检测阳性１９例（３２．２％）ＥＲ阳性者多见于≥５０岁者和ＢｏｒｒｍａｎｎⅢ、Ⅳ型者。ＥＲ阳性者ＤＩ值较低，以二倍体较多见，而ＥＲ阳性异倍体胃癌淋巴结转移和浆膜浸润多见。结果表明：胃癌组织中存在雌激素受体，联合检测ＥＲ和ＤＮＡ可为胃癌内分泌治疗和预后评估提供更多的生物学依据。

ER was detected in 59 cases of paraffin-embedded tissues of gastric cancer by the mothed of histo-chemical affinity half-quantity.56 cases were measured by FCM for DNA content. ER was found positive in 19 cases(32.2%).The ER positive rate is significantly higher in patients≥50 years old and Borrmann 、. In ER positive group, the DI was lower and the incidence of diploid was higher. And ER positive cases with aneuploidy had a higher incidence of lymph node and serosa involvement.The results suggest that there is ER in tissues of gastric cancer,simultaneous detection of ER and DNA content in gastric cancer can provide biological foundation for its clinical endocrine treatment and evaluation of prognosis.