脂肪肝家鸭模型的建立及建模条件优化

Establishment and Optimization of an Animal Model for Fatty Change of Liver

目的建立基本符合脂肪肝临床发病特征的家鸭脂肪肝模型,并对建模条件进行优化。方法建立两种家鸭脂肪肝模型并进行对比分析。两种模型分别为短时间(28d)高剂量高糖饮食(100 g.d-1)法(模型I)和长时间(49d)低剂量高糖饮食(50 g.d-1)法(模型Ⅱ),通过考查体重、血清脂质、血糖、肝脏脂质、肝脏病理形态学等指标进行评价。结果两个模型组家鸭体重、肝指数、血清胆固醇(TC)、甘油三酯(TG)、血糖均高于空白对照组,且存在大量肝细胞脂肪变性,模型Ⅰ组肝TG高于空白对照组。结论两种方法均可建立脂肪肝家鸭模型,模型I优于模型Ⅱ。

Objective To establish and optimize an animal model for fatty change of liver with clinical characteristics. Methods Comparative analysis of two kinds of duck fatty change model of liver was established. The ducks of model Ⅰ group were fed with high-dose high glucose diet for 28 days. The ducks of model Ⅱ group were fed with low-dose high glucose diet for 49 days. The index of body weight, ratio of liver weight/body weight, blood sugar serum grease, liver grease, liver pathology was observed. Results The body weight, ratio of liver weight/body weight, serum cholesterol, serum triglyceride and blood sugar of two model groups were higher than that of the control group. Pathological alteration of hepatocyte steatosis in liver was observed in both model group. The liver triglyceride of model Ⅱ group was higher than that of the control group. Conclusion Two methods can replicate the fatty change model of liver successfully, but the model Ⅰ is better than the model Ⅱ .