培垛普利和依那普利拉对大鼠胸主动脉内皮依赖性影响

Endothelium dependent effect of perindopril and enalaprilat in rat thoracic aorta 1

培垛普利和依那普利拉对大鼠胸主动脉内皮依赖性影响１房振英，李雅菲，张丽丽，刘达谨，李运乾（山西省心血管病研究所，太原０３０００１，中国）关键词培垛普利；依那普利拉；内皮获得性释放因子；胸主动脉目的：研究血管紧张素转换酶（ＡＣＥ）抑制剂培垛普利（Ｐｅｒ...

IM: To study the effect of the angiotensin converting enzyme (ACE) inhibitors perindopril (Per) and enalaprilat (Ena) on the reactivity of the endothelium in normal rats. METHODS: Male rats were treated intragastrically with Per (2 mg·kg -1 ·d -1 ) or placebo ( n =18) for 6 wk. Aorta was isolated for experiment. Another set of isolated aortic rings with and without endothelium were incubated with Ena (0 1 μmol·L -1 ) for 30 min. Responses to acetylcholine, serotonin, phenylephrine, sodium nitroprusside (SN), and nitroglycerin (Nit) were observed. RESULTS: Endothelium dependent relaxation to acetylcholine was augmented in aortic rings from rats treated with Per in comparison with control. The IC 50 value (95 % confidence limits) decreased from 3 8 (0 56-26 1) μmol·L -1 (control group) to 0 98 (0 28-3 41) μmol·L -1 (Per treated group). The maximal relaxation was augmented from 62±9 % to 78±10 % ( P <0 01) . How￣ever, the responses to the endothelium independent vasodilators, SN and Nit, were similar. Serotonin and phenylephrine induced contractions were decreased, which were influenced by basal release of endothelium derived relaxing factor (EDRF). EC 50 value was 6 1 (2 6-14 4) nmol·L -1 vs 8 3 (3 6-18 8) nmol·L -1 in comparison with control group and Per treated group. The maximal contraction was decreased from 2 42±0 29 g (control group) to 1 96±0 25 g (treated group) ( P <0 01). Similar results were found in incubation with Ena. CONCLU￣SION: Ena and Per enhanced the basic release of EDRF from vascular endothelium.