尖吻蝮蛇毒和蝮蛇毒凝血酶样酶促凝的协同作用

Synergism of procoagulation effect of thrombin like enzymes from Dienagkistrodon acutus and Agkistrodon halys snake venoms

尖吻蝮蛇毒和蝮蛇毒凝血酶样酶促凝的协同作用魏文利１，孙家钧，陈家树（中山医科大学药理教研室，广州５１００８９，中国）关键词尖吻蝮蛇；蝮蛇；蛇毒；凝血酶类；血液凝固目的：尖吻蝮蛇（ＤＡ）和蝮蛇（ＡＨ）蛇毒凝血酶样酶（ＴＬＥ）对促凝作用的协同效果．方法：...

IM: To study the synergistic effect of thrombin like enzymes (TLE) of Dienagkistrodon acutus (DA) and Agkistrodon halys (AH) venoms. METHODS: TLE were isolated from venoms of DA and AH by successive column chromato￣graphy. Effects of combination of DATLE and AHTLE and related factors on the clotting time and clot quality were tested in vitro . RESULTS: One coagulation unit was 2 7 μg for DATLE and 304 4 μg, for AHTLE with reference standard of thrombin (42 2 μg). TLE induced clot was a fibrin monomer which was fragile and did not retract. Combination of AHTLE and DATLE shortened the clotting time and decreased the solubility of the clot in urea 5 mol·L -1 . When subthreshold concentration of thrombin or physiological concentration of Ca 2+ was added, the clotting time was further shortened, the clot was no longer soluble in urea and retracted well, and the resistance of the clot to plasmin degradation was increased. CONCLUSION: A synergistic effect of DATLE and AHTLE accelerated hemocoagulation and improve clot quality.