铜污染对高羊茅生长及活性氧代谢影响的研究

Effects of Cu on Growth and Activate Oxygen Metabolism of Festuca arundinacea

通过水培实验研究了重金属铜对高羊茅生长及活性氧清除系统的影响。结果表明低浓度Cu（〈10mg／L）对高羊茅植株生长无明显抑制现象，在Cu浓度低于5mg／L时，甚至起促进作用。与对照组相比，低浓度cu处理组植株干重、鲜重及叶绿紊含量均略微升高，而MDA水平及O2·-产生速率变化不显著。SOD、CAT和POD活性显著升高，保护酶系统仍保持平衡。但随Cu浓度（10～40mg／L）增加则显示出一定的负效应，与对照组相比，高羊茅植株矮小，叶片发黄，根短且数目少，植株干重、鲜重和叶片色素含量均显著降低，并且随Cu浓度的增加而变化显著。同时,随Cu浓度增加，O2·-产生速率急剧增加，叶片细胞膜透性增大，电导率显著升高．MDA水平上升，且活性氧清除系统遭到破坏，保护酶系统失衡。研究同时显示，高羊茅是一种有潜力于Cu污染土壤修复的草坪草。

The effects of copper pollution on the seedling growth and activate oxygen metabolism of F. arundinacea were studied by water cultivation experiments. The results showed that under low concentration of Cu（（10 mg/L）, there were no remarkable suppression on the growth of F. arundinacea, furthermore,when the concentration of Cu lower than 5 mg/L, the growth of F. arundinacea could be improved, their fresh or dry weight and the content of chlorophylls in leaves increased, while the velocity of O2 · - production and the content of MDA had no marked change, the activities of SOD, POD or CAT increased, and the balance of protective enzyme system was hold. However, with the increase of Cu concentration （10-40 mg/L）, negative effect had existed, the fronds were shorter and smaller, the fresh/dry weight and content of leaf pigments decreased drastically, the roots were shorter and fewer. The velocity of O2 ·- production was increased ,sharply, the membrane penetration, electric conductivity and MDA content of leaf cells were increased. Furthermore, the activate oxygen metabolism system of F. arundinacea was destroyed, the balance of protective enzyme system was broken. The research showed in the meantime, F. arundinacea was a kind of turfgrass that had a potential in restoration for soil polluted by Cu.