糖尿病性白内障大鼠晶状体谷胱甘肽含量的变化

Study on relevance of glutathione with diabetic cataract

目的:分析谷胱甘肽和糖尿病性白内障的相关性。方法:复制链脲佐菌素(streptozocin,STZ)糖尿病大鼠模型,成模后采用裂隙显微镜观察并记录晶状体混浊的程度,使用二硫硝基苯法测定糖尿病性白内障大鼠晶状体中谷胱甘肽的含量。结果:与正常大鼠相比糖尿病性白内障大鼠晶状体中谷胱甘肽的含量降低70%(P<0.05),它们之间差异具有统计学意义。并且糖尿病性白内障大鼠晶状体中谷胱甘肽的含量与它的晶状体混浊程度呈负相关(rs=-0.783)。结论:随着糖尿病性白内障的发生和进展,晶状体中的谷胱甘肽含量明显减少。

AIM： To analyze the relevance of glutathione with diabetic cataract. NETHODS： Diabetic cataract was induced by streptozocin （STZ）. Progression of lens opacification was recorded using slit lamp at regular time intervals. The levels of glutathione （GSH） in the lens were determined spectrophotometrically.RESULTS： The level of GSH in the lens of the diabetic rats was significantly lower compared with normal rats （70%, normal rats vs diabetic rats ,P〈0.05）. The level of GSH in the lens of the diabetic rats was negative correlation with the lens opacification （P 〈 0.05, rs= - 0.783）. CONCLUSION： The level of GSH in the lens is obvious decreased with the development of the diabetic cataract. The decreased GSH may contribute to the formation of diabetic cataract.