摘要
目的建立中药三七的高效液相色谱特征谱。方法运用加压溶剂提取、HPLC-DAD分析28个不同产地的三七药材,所得的图谱采用中国药典委员会开发的“中药色谱指纹图谱相似度评价系统(Version 2004A)”软件进行分析。结果28个三七样品色谱图中各色谱峰分离良好,从中确定了13个特征峰,其中8个主要色谱峰经对照品确认,所有药材色谱图与软件生成的对照谱相似度为0.982±0.008(RSD=0.78%)。结论该方法简便、重现性好、具可操作性,可科学评价及有效控制三七药材质量。
Aim To develop the chemical characteristics of Panax notoginseng in order to control its quality. Methods The samples was extracted using pressurized liquid extraction （PLE） and analyzed by HPLC-DAD. The data were evaluated using the ＂ similarity evaluation system for chromatographic fingerprint of TCM＂ software （ version 2004A）. Results The chromatographic characteristics of 28 Panax notoginseng from different places were established using HPLC-DAD, and 8 peaks among 13 typical ones in the chromatograms were identified by comparing with their reference compounds. The similarity of different samples was high （0. 982 ±0. 008, RSD =0.78% ）. Conclusion The chromatographic characteristics are useful to control the quality of Panax notoginseng.
出处
《药学学报》
CAS
CSCD
北大核心
2006年第11期1090-1093,共4页
Acta Pharmaceutica Sinica
基金
澳门大学科研基金(RG029/04-05S/C86toS.P.Li)
关键词
三七
人参皂苷
加压溶剂提取
相似度评价
Panax notoginseng
ginsenoside
pressurized liquid extraction
similarity evaluation
